PHYTOHAEMAGGLUTININ

PHAand HRP Conjugated PHA Antibody Will Appear Blue In Color And Then Turn Yellow After The Addition Of The Stop Solution. The Optical Density (OD) Is Measured Spectrophotometrically At A Wavelength Of 450 Nm. The OD Value Is Proportional To The Concentration Of PHA. You Can Calculate The Concentration Of PHA In The Samples By Comparing The OD Of The Samples To The Standard Curve